My quad has bicycle grade wheels/tires on it right now and I have taken it to 70 mph thus far, uphill(I don't know what it will actually do yet). They are not adequate for the task, but they didn't fail on me, either. I have had to re-true the rear wheels already. Planning to have DOT grade parts on it again soon.
A good set of Alex or Velocity USA double-walled aluminum bicycle rims with Schwalbe Marathon Plus tires, I'd trust for 35 mph cruising speeds on bad roads using the bike as a daily commuter for years without any trouble. I've done it before with them for thousands of miles at a time between repairs or tire/tube changeouts. Those Alex or Velocity USA rims aren't DOT compliant, but their strength to weight ratio far exceeds any DOT rim I've tried. In the case of the Velocity USA rims, I put over 40,000 miles on them before they cracked around the spoke nipples and I had to replace them. Aluminum does fatigue, and that's what sucks about it.
If your vehicle is light enough, you might be able to get away with a combination of 20" bicycle rims of sufficient strength, with 16" DOT tires shoehorned onto them, even at 50 mph cruising speeds. Lace them bicycle rims with 13ga or 14ga spokes for best possible strength, and check them frequently.