I haven't worried about a physical separation between the two plastic "wrappers" unless the voltage difference is more than one cell in which case I simply add a layer of duct tape. I do glue the cells together with a couple of thin strips of polyurethane glue, and I typically opt for a staggered alignment instead of square for the minimum height as well as a stronger glued structure (each cell touches more cells).
I've received a lot of pre-made blocks of cells that are hot glued together, and hot glue doesn't work worth a damn on those plastic sleeves. It may seem like it holds at first, but it's not a good bond. I've pulled off hundreds of hot glue strips without damaging a single fragile plastic wrapper. I don't want my battery structure supported just by the tab welds, because then vibrations can cause movement between cells opening the door for different types of failures.
If you ever plan to solder something to the tabs near the button end, then yes add paper O-rings before tab welding. The plastic ones don't seem to have a very high melting point.
For great flexibility in pack shape, using building blocks of 5p or 7p in a trapezoid shape works great...like this
The red X's were to show my agent in China to make sure the tab doesn't extend into those spaces, which could cause issues later when stacking. The button end gets and extra few cm of tab consistently off to the same side, so you're soldering or welding that extra tab to the flat cell end of the next block.
Trapezoid blocks work great for making a cylindrical pack by making your series connections end-to-end forming one half, and then back, so the pos and neg terminals are at the same end of the cylinder. Plan your series connections very well ahead of time. With today's high capacity 18650's 5p trapezoids end-to-end wrapped in glass cloth, and then 2 strings of those wrapped in carbon to form the main tubes of an ebike sure is interesting to me. 8)