Yes. See my CrazyBike2 stuff for some pictures of the wiring (they're not specifically of the wiring, but show it here and there).
Mine are the 60-amp Andersons, I think; they're a bit hard to push together to connect, and take some force to pull apart, so they don't just come undone.
I have a connector on each battery, and those connect to a harness wired so that they all end up in series with a breaker and an ammeter at the most negative end of the string, between it and the bike frame (which is "ground" because of the way the old incandescent Honda scooter turn signals I use mount, and use their case/mount bolt for ground).
I've never had a connector come loose, even when my pants seams rub on them or in one case got caught on one that had it's ziptie-holddown break, and was repeatedly pulled on as I pedalled for some distance until I could pull over and fix the ziptie.